Have you ever wondered whether your screening results are skewed by poor library representation or uneven distribution bias from the start? The solution is an integrated, end-to-end workflow that maintains library fidelity from synthesis through cell pool generation.
 
We recently completed a mouse genome-wide knockout screening project, Here are the key results:
1. Library plasmid construction
    • Library representation: 99.95% (target >99%)
    • Distribution uniformity: 4.0 (<10 requirement)
2. Lentivirus production
    • Viral titer: ≥1×108TU/mL
    • Puromycin selection (10μg/mL) eliminated non-transduced cells within 48 hours
3. sgRNA library cell pool construction
    •  Final cell pool coverage: 97.93% (>85% target)
    •  Distribution uniformity: 8.8 (<15 requirement)
 
Behind these results is our fully integrated workflow from library synthesis and lentivirus packaging to cell pool construction, ensuring consistency, traceability, and reproducibility at every step.
 
Ready to make your functional genomics screen more robust and hassle-free? Let’ s discuss how we can support your next discovery.

   
 
 
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Synbio Technologies LLC
9 Deer Prk Dr., Suite J-25, Monmouth Junction, NJ 08852, USA