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At Synbio Technologies, we provide high-efficiency
Lentiviral Packaging Services
to accelerate gene function research and cell line development. I am excited to share with you a case study that demonstrates our expertise in vector design, viral packaging, and stable cell line establishment.
Case Overview
THP-1 suspension cells
are challenging due to their low transduction efficiency and sensitivity to conditions. To achieve precise BCL2A1 gene silencing, we optimized a high-titer lentiviral vector system for successful gene delivery, stable knockdown, and reliable cell line development.
Challenges & Solution
Precision-Verified Constructs
– Synthesized and validated BCL2A1-shRNA with 100% accuracy.
High-Titer Lentivirus Production
– Efficient 293T transfection and optimized purification for maximum viral yield.
Stable Cell Line Establishment
– Puromycin selection for consistent and long-term gene knockdown.
We established a stable THP1 cell line targeting BCL2A1 knockdown and determined the optimal selection concentration of puromycin to eliminate untransfected cells.
If you're looking for a reliable lentiviral packaging partner, we’re here to help.
Let’s discuss how we can support your next project!
Additionally, our VIP customers enjoy access to a range of premium services：
•
Gene Synthesis
•
Adenovirus Packaging
•
RNAi Synthesis
•
Adeno-associated Virus Packaging
•
Gene Editing
•
Vector Libraries
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Synbio Technologies LLC
9 Deer Prk Dr., Suite J-25, Monmouth Junction, NJ 08852, USA