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Various types of DNA libraries provide technical support for breakthrough research such as protein directed evolution, drug target screening, specific antibody discovery, and more. Based on our advanced design and leading manufacturing processes, Synbio Technologies has successfully delivered hundreds of high-quality, customized, and accurately synthesized DNA libraries. The types of libraries include precise variant libraries, CRISPR sgRNA libraries, human genome CRISPR knockout libraries, and antibody libraries. We provide DNA libraries of various sizes and complexities at a competitive delivery speed to ensure the efficient development of downstream library screening and gene function research.
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| 1. Precise Variant Libraries |
| Degenerate Mutation Library |
Trimer Library |
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The NNK library is a classic degenerate mutation library. The NNK degenerate primers used contain 32 (4x4x2) codon combinations (N= A/C/G/T, K= G/T), covering all 20 amino acids, and can saturate amino acids at any site.
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Trimer libraries can customize each site to be composed of different ratios of amino acids. Among the four Trimer sites, for example, the X1 Trimer site is A (80%): G (3%) : R (3%) : S (3%): T (8%) : V (3%).
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| Fig.1 The proportion of each codon contained in the five NNK sites in the NNK library |
Fig.2 Types and proportions of custom amino acids |
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| 2. CRISPR sgRNA Libraries |
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Synbio Technologies provides one-stop gene function screening service based on CRISPR sgRNA library. The Syno® HT high-throughput synthesis technology platform can synthesize hundreds of thousands or even millions of sgRNAs on one chip at the same time, to quickly deliver high-quality sgRNA libraries at an affordable cost.
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| Customized CRISPR sgRNA Libraries |
Human Genome CRISPR Knockout Libraries |
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• Professional Design Center: Single sgRNA, multiple sgRNA and sgRNA library design services.
• Experienced: Well-designed sgRNA sequences with the lowest off-target rate. Our dedicated team provides professional analysis to ensure the success of every project.
• Applicable in Many Species: Our database covers sgRNA design for >20 different species; Specific sgRNA design is also available upon request for certain species.
• Industry Leader in Manufacturing Standards: We've successfully delivered 200+ customized high-quality libraries.
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