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Based on advanced design and leading manufacturing process, Synbio Technologies has successfully delivered hundreds of high-quality, customized, and accurately synthesized DNA libraries. The types of libraries include oligo pools, precise mutant libraries(degenerate mutation library, Trimer library), and genome editing libraries (CRISPR-Cas9 sgRNA library, human genome CRISPR knockout library), antibody libraries, etc. We provide DNA libraries of various size and complexity at a competitive delivery speed to ensure the efficient development of downstream library screening and gene function research.
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Competitive Advantages
• Highly Customized Strategy: Exactly what you need.
• Cost-Effective Solutions: Reduces difficulty of downstream screening.
• Extensive Experience: Hundreds of libraries have been successfully delivered.
• One-Stop Services: Design, synthesis, bio-analysis, etc.
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Case Studies of Trimer Libraries
Precise mutant libraries designed with the trimer mixture as the element, also known as trimer libraries, can ensure that the amino acids at the mutation site are composed according to the designed ratio to meet the amino acid diversity and codon preference of the specific site. Trimer libraries are an efficient solution for complex libraries and can further improve the accuracy of library screening.
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1. Trimer libraries can be used for site saturation mutation to distribute 20 amino acids in equal proportion, further reducing codon redundancy and removing termination codons in the library.
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Fig.1 Trimer libraries are also a saturated mutation library with equal proportion distribution
of each amino acid.The proportion of each codon contained in the five sites. |
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2. Trimer libraries can also customize each site to be composed of different ratios of amino acids. Among the four Trimer sites, for example, the X1 Trimer site is A (80%) : G (3%) : R (3%) : S (3%): T (8%) : V (3%).
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| Fig.2 Types and proportions of custom amino acids. |
Fig.3 Ratio between actual and theoretical proportion |
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of each amino acid. |
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Related Services
Gene Synthesis
Synthetic DNA Libraries
DNA Sequencing & Gene Analysis
Protein Expression
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