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With the worldwide outbreak and spread of COVID-19, there is an urgent need to develop a rapid and accurate diagnostics for SARS-CoV-2 infection. At present, SARS-CoV-2 laboratory diagnosis mainly includes nucleic acid tests, antigen immunology tests, serum antibody immunology tests, or Luminex multi-factor tests (Fig.1). Nucleic acid detection technology, based on a molecular level, has gradually become the mainstream method of pathogen detection. It can be directly detected without the need for pathogen isolation and culture in advance, which is convenient, fast, and has higher sensitivity. Currently, we have developed a variety of nucleic acid detection technologies, and the realization of these technologies cannot be achieved without "probes". Probes are widely used in different nucleic acid detection techniques (Tab.1).
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| Fig.1 Molecular structure and laboratory detection method of SARS-CoV-2 |
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| Probes
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Laboratory Tests |
Applications
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• TaqMan
• MGB
• LNA Probe
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RT-PCR
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Specific primers and TaqMan probes were designed for the conserved regions of ORF1Ab and N genes, two independent genes of 2019-nCoV virus. |
• Fluorescent
• Quencher
• THF
• Blcoker
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RT-RPA
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RT-RPA combines isothermal reverse transcription, using recombinases to catalyze the entry of primer strands into dsDNA. RPA amplification products can also be detected quantitatively by RT-RPA probes. |
• FQ reporter Probe
FAM-UUUUU-BHQ
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CRISPR-Cas RNA
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Novel Coronavirus probes for nucleic acid detection based on CRISPR/ Cas13A, which include Cas13A and its buffer, the gRNAs, and fluorescent reporter molecules. When a gRNA complements with the conserved target gene of SARS-CoV-2, Cas13A is activated to cut off the fluorescence reporter molecule in fluorescence quenching and generates a fluorescence signal. |
• Methylene Blue
• Ferrocene
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Electrochemical Sensor
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The nucleic acid of the novel coronavirus is specifically identified by probes, and the signal molecules are enriched by nanomaterials, so as to play the role of signal amplification and realize the portable and quick detection of novel coronavirus on the smartphone terminal. The biosensor consists of a capture probe, a target sequence, a labeled probe and an auxiliary probe. |
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| Tab.1 Applications of probes in different nucleic acid detection techniques |
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It can be seen that probes play an important role in nucleic acid detection. Synbio Technologies can provide the services such as qPCR probes, capture probes, FISH probes and other molecular diagnostic materials based on the above nucleic acid detection technologies.
Synbio Technologies can provide a full range of RT-qPCR fluorescence and quenchers. At the same time, based on different detection principles, our powerful modification abilities can complete different types of probes, such as: TaqMan/MGB/BHQ, RAA/RPA Probes, Molecular Beacons, Scorpions Probes, LNA Probes, Dual Quencher Probes, etc. No matter how complex the synthetic sequences are, we can always meet the needs of our customers. All of these services are conducted under ISO 9001 & ISO 13485 quality management system standards, which can meet the demands of scientific research and industries from R&D to production.
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Competitive Advantages
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• High Purity Guarantee: Made with refined purification technology, the probes have high purity and low background noise.
• Quality Assurance: Efficient removal of small molecule fluorescent dye residues, and 100% LC-MS quality verification for each probe.
• Excellent Stability: ISO 9001& ISO 13485 quality control management systems ensure control, traceability, and batch-to-batch consistency during the entire process.
• Highly Customized: Various synthetic specifications from nmol to umol, HPLC, PAGE, and other purification methods with high purity and high standards are provided.
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