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  June 2015
Products News Case Study Events Recommended Contact Us
 

Desktop Sequencer BGISEQ-500
Shenzhen, China, June 27, 2015-- BGI today debuted its desktop sequencer BGISEQ-500 in China. It is the second new system based on Complete Genomics’ industry-leading sequencing technology. Earlier this month, CG's new fully integrated “supersequencer” Revolocity™ system was previewed at ESHG. Different from Revolocity™’s scalability, BGISEQ-500 is an integrated desktop sequencing solution. It automates sample preparation, sequencing, and data analysis, enabling various applications on one-click operation.

Revolocity™ Sequencing System
MOUNTAIN VIEW, Calif. – June 6, 2015 – Complete Genomics, Inc., a leader in whole human genome sequencing, today announced that Mater Health Services, a leading Australian health services provider, and Radboud University Medical Center in the Netherlands are to become the first customers for the Revolocity™ sequencing system, the company’s new fully integrated “supersequencer.” The Revolocity system is being previewed at the European Human Genetics Conference (June 6–9, Scottish Exhibition and Conference Centre [SECC], Glasgow, UK) on June 6, 2015.

Whole genome resequencing
By comparing the genome sequences at the individual or population level, genomic variations can be analyzed. Molecular breeding based on resequencing includes a variety of applications, such as functional gene or marker detection, functional characterization of genes, and new varieties development. To date, we have successfully completed the Silkworm, Maize, and Soybean Projects, and have gained much experience from many other on-going plant and animal resequencing projects.

Bisulfite Sequencing
Bisulfite Sequencing is a methodology that uses bisulfite treatment combined with high-throughput sequencing to draw the most complete picture of a DNA methylome. BS is currently the sole method that can attain both single-base resolution and genome-wide coverage. It has been successfully applied by BGI for elucidation of the methylomes of human cells as well as of other species such as silkworm, rat, mouse, Arabidopsis, zebra fish, maize, rice, bee, chicken, and so on.

Metagenomic Sequencing
Metagenomics is the study of genomes contained within an entire microbial community. This technology has opened up a new era in the study of microbial diversity with direct access to the genomes of numerous non-cultivatable microorganisms in their natural habitat. Metagenomic sequencing analyzes microbial community diversity, gene composition and function, as well as metabolic pathways associated with the specific environment. This approach has been applied to environmental studies as well as biomarker research.

Genome sequence of foxtail millet (Setaria italica) provides insights into grass evolution and biofuel potential
Nature Biotechnology 30, 549–554 (2012) doi:10.1038/nbt.2195
Foxtail millet (Setaria italica), a member of the Poaceae grass family, is an important food and fodder crop in arid regions and has potential for use as a C4 biofuel. It is a model system for other biofuel grasses, including switchgrass and pearl millet. We produced a draft genome (~423 Mb) anchored onto nine chromosomes and annotated 38,801 genes. Key chromosome reshuffling events were detected through collinearity identification between foxtail millet, rice and sorghum including two reshuffling events fusing rice chromosomes 7 and 9, 3 and 10 to foxtail millet chromosomes 2 and 9, respectively, that occurred after the divergence of foxtail millet and rice, and a single reshuffling event fusing rice chromosome 5 and 12 to foxtail millet chromosome 3 that occurred after the divergence of millet and sorghum. Rearrangements in the C4 photosynthesis pathway were also identified.

The DNA Methylome of Human Peripheral Blood Mononuclear Cells
PloS Biology. DOI: 10.1371/journal.pbio.1000533
DNA methylation plays an important role in biological processes in human health and disease. Recent technological advances allow unbiased whole-genome DNA methylation (methylome) analysis to be carried out on human cells. Using whole-genome bisulfite sequencing at 24.7-fold coverage (12.3-fold per strand), we report a comprehensive (92.62%) methylome and analysis of the unique sequences in human peripheral blood mononuclear cells (PBMC) from the same Asian individual whose genome was deciphered in the YH project. PBMC constitute an important source for clinical blood tests world-wide. We found that 68.4% of CpG sites and <0.2% of non-CpG sites were methylated, demonstrating that non-CpG cytosine methylation is minor in human PBMC. Analysis of the PBMC methylome revealed a rich epigenomic landscape for 20 distinct genomic features, including regulatory, protein-coding, non-coding, RNA-coding, and repeat sequences. Integration of our methylome data with the YH genome sequence enabled a first comprehensive assessment of allele-specific methylation (ASM) between the two haploid methylomes of any individual and allowed the identification of 599 haploid differentially methylated regions (hDMRs) covering 287 genes. Of these, 76 genes had hDMRs within 2 kb of their transcriptional start sites of which >80% displayed allele-specific expression (ASE). These data demonstrate that ASM is a recurrent phenomenon and is highly correlated with ASE in human PBMCs. Together with recently reported similar studies, our study provides a comprehensive resource for future epigenomic research and confirms new sequencing technology as a paradigm for large-scale epigenomics studies.
 
BGI Participated in Series of Sino-Italian Tumor Workshops
BGI Attending Milan EXPO 2015 and Outlining New Agricultural Progress
B10K - Toward decoding all bird genomes

PAG Asia Singapore 7.13-15
ISPD Washington, USA 7.12-15

Birds are the most species-rich class of tetrapod vertebrates, with over 10,000 living species distributed in diverse niches all over the world. Birds are widely used as models for investigating evolutionary and ecological questions. Resolving phylogenetic relationships of Neoavian species and estimating divergence times have been a huge challenge for scientist. One for the challenge is that it has been proposed, but not proven, that to be that there was a ‘big bang’ radiation for Neoavian birds, where many species are closely related at the dawn of the radiation. This radiation could theoretically be solved with whole genomes. However, by 2010, only three species (chicken, turkey and zebra finch) had whole genome sequences. Because of the lack of genomic data, many other avian-related questions could also not be addressed systematically.


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